Quantitative radioimmunohistochemical measurements of p185(erbB-2) in frozen tissue sections.

Accuracy of Frozen Sections

Background and Objective: Intraoperative consultation by frozen section is a high – risk procedure with important consequences. Therefore, it is critical to determine efficiency of frozen section performance periodically. This study was performed to determine the accuracy of frozen section in Urmia University of Medical Sciences. Materials & Methods:Inthis cross sectional study, we compared th...

Study of vitrified, unstained frozen tissue sections by cryoimmunoelectron microscopy.

We describe the development and application of a novel approach to high-resolution ultrastructural analysis of cells and tissues. It is based on the preparation of ultrathin frozen sections of fixed tissues, rinsing of the sections, followed by their embedding on the grid in a layer of vitrified ice, and direct observation with a cryoelectron microscope. Examination of smooth muscle, kidney and...

The Use of indirect Immunoperoxidase Assay in Diagnosis of Type A (H9N2) Avian Influenza Virus Antigen on Frozen Tissue Sections

accuracy of frozen sections

background and objective: intraoperative consultation by frozen section is a high – risk procedure with important consequences. therefore, it is critical to determine efficiency of frozen section performance periodically. this study was performed to determine the accuracy of frozen section in urmia university of medical sciences. materials & methods:inthis cross sectional study, we compare...

Ultrathin Frozen Sections

A relatively simple method for obtaining ultrathin, frozen sections for electron microscopy has been developed. Tissues, cultured cells, and bacteria may be employed. They are fixed in 1.25-4% glutaraldehyde for 1-4 hr, are washed overnight in buffer at 3 degrees C, and are embedded in 20% thiolated gelatin or pure gelatin. Before sectioning they are partially dehydrated in 50% glycerol, frozen...